FIGURE 4.

Immunoblot analysis of the activation of ERK1/2 and p38MAPK in resting and activated microglia. (A) Activation of ERK1/2 upon DA stimulation in resting and activated Bv2 microglia. Total cell lysates were collected at various time points (5, 10, and 30 min) after DA (2 μM) stimulation. The lysates were separated by SDS-PAGE, transferred onto PVDF membranes, and immunoblotted with the anti-phospho-ERK1/2 antibody. Immunoblots with anti-ERK1/2 antibody were used for loading control. ERK1/2 were measured as fold increase of the control. Values are means ± standard error of three or four independent experiments. (B) Activation of p38MAPK in resting, but not in activated microglia in response to 2 μM of DA. Both the resting and activated BV₂ microglia were treated with 2 μM of DA for 30 min. Total cell lysates were separated by SDS-PAGE and immunoblotted with an anti-phospho-p38MAPK antibody. Values are means ± standard error of four independent experiments. (ANOVA: ^∗P < 0.05, ^∗∗P < 0.01 compared to control).