FIGURE 7.

(A) Phosphorylation of paxillin at Ser⁸³ via the activation of p38MAPK in resting microglia in response to DA. Paxillin phosphorylation was detected by immunoblots with the anti-phospho-paxillin Ser⁸³ antibody. Immunoblots with anti-paxillin antibody were used as a loading control. Representative images of western blots for the phosphorylation of paxillin at Ser⁸³ are shown. Values are means ± standard error of four independent experiments (ANOVA: ^∗P < 0.05). (B) Inhibition of Ser⁸³ phosphorylation by the p38MAPK inhibitor. Resting Bv2 microglia cells were pre-treated with 0.5 μM SB203580 (p38MAPK inhibitor) for 10 min and then 2 μM DA for 30 min. Values are means ± standard error of three independent experiments (ANOVA: ^∗P < 0.05). (C) DA downregulates paxillin phosphorylation at Ser⁸³ in activated microglia (ANOVA: ^∗∗P < 0.01).