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. 2018 Sep 11;12:304. doi: 10.3389/fncel.2018.00304

Figure 1.

Figure 1

All dorsal root ganglion (DRG) neurons express calcium-dependent activator protein for secretion 1 (CAPS1) while only 50% express CAPS2. (A) CAPS1 and, to a lesser extent, CAPS2 mRNA was detected in DRGs. Total RNA from the DRGs (five adult mice) and cerebellum as positive control was probed for CAPS1 and CAPS2 by RT-PCR. (Bi) Western blot (WB) analysis of CAPS protein expression during development. DRGs were isolated from wild-type (WT) and CAPS DKO E18, and WT P7 mice and blotted with the indicated antibodies (Table 1). Actin was used as a loading control. (Bii) Actin-based corrected protein expression levels were normalized to the P7 signal (n = 2). (Ci) Immunocytochemistry of DRG neurons in culture with anti-CAPS1 (top) and anti-CAPS2 (bottom) antibody suggests that CAPS1 is present in all DRG neurons while CAPS2 is present in a subpopulation of DRG neurons. The specificity of the antibodies was verified (Supplementary Figure S5). (Cii) Quantification of CAPS paralog expression among DRG neurons (Nmice = 5, nneuron = 70 and 191 for CAPS1 and CAPS2, respectively).