Table 1.
Antibody | Host | Immunogen | Manufacturer and catalog no. | Working dilution |
---|---|---|---|---|
Primary antibody | ||||
CAPS1 | Rabbit | Recombinant protein (aa 18–107 of mouse CAPS1) | Synaptic systems (No. 262 013) | 1:1,000 and 1:500 |
CAPS2 | Rabbit | Full length CAPS2e. This antibody was immuno-purified against a CAPS2 specific sequence GSGGGAARPV | Provided by M. Jung | 1:1,500 |
Synapsin | Guinea pig | Synthetic peptide: aa 2–28 (rat) | Synaptic systems (No. 106 004) | 1:1,000 |
Bassoon | Rabbit | Recombinant protein aa 330—C terminal (rat) | Synaptic systems (No. 141 003) | 1:300 |
PSD95 (monoclonal) | Mouse | Fusion protein aa 77-299 (human) | NeuroMab (Clone K28/43) | 1:500 |
eGFP | Rabbit | Full length protein | Life technologies (G10362) | 1:20 |
Chromogranin A | Rabbit | Recombinant fragment from the C-terminal (human) | Abcam (ab15160) | 1:1,000 |
NF200 | Mouse | C-terminal tail segment of dephosphorylated NF200 (H) subunit | Sigma Aldrich (N0142) | 1:5,000 |
Substance P/TAC1 | Rabbit | Human TAC1-GST fusion protein (Ag4790) | Proteintech (13839-1-AP) | 1:200 |
β-actin | Mouse | Monoclonal, clone AC-15 | Sigma Aldrich (A1978) | 1:5,000 |
Secondary antibody | Life technologies, Invitrogen | |||
Alexa 488 | Goat anti-mouse | A-11001 | 1:2,000 | |
Alexa 488 | Goat anti-guinea pig | A-11073 | 1:2,000 | |
Alexa 568 | Goat anti-mouse | A-11004 | 1:2,000 | |
Alexa 647 | Goat anti-mouse | A-21235 | 1:2,000 | |
Alexa 568 | Goat anti-rabbit | A-11011 | 1:2,000 | |
Alexa 488 | Goat anti-rabbit | A-11008 | 1:2,000 | |
Alexa 647 | Goat anti-rabbit | A-21244 | 1:2,000 | |
Fab fragments | Goat anti-mouse (IgG H&L) | Rockland inc. (810–1102) | 1:50 | |
STAR Red | Goat anti-rabbit (IgG) | Abberior (2-0012-011-9) | 1:100 |