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. 2018 Sep 11;12:304. doi: 10.3389/fncel.2018.00304

Table 1.

Details of antibodies used in the study.

Antibody Host Immunogen Manufacturer and catalog no. Working dilution
Primary antibody
CAPS1 Rabbit Recombinant protein (aa 18–107 of mouse CAPS1) Synaptic systems (No. 262 013) 1:1,000 and 1:500
CAPS2 Rabbit Full length CAPS2e. This antibody was immuno-purified against a CAPS2 specific sequence GSGGGAARPV Provided by M. Jung 1:1,500
Synapsin Guinea pig Synthetic peptide: aa 2–28 (rat) Synaptic systems (No. 106 004) 1:1,000
Bassoon Rabbit Recombinant protein aa 330—C terminal (rat) Synaptic systems (No. 141 003) 1:300
PSD95 (monoclonal) Mouse Fusion protein aa 77-299 (human) NeuroMab (Clone K28/43) 1:500
eGFP Rabbit Full length protein Life technologies (G10362) 1:20
Chromogranin A Rabbit Recombinant fragment from the C-terminal (human) Abcam (ab15160) 1:1,000
NF200 Mouse C-terminal tail segment of dephosphorylated NF200 (H) subunit Sigma Aldrich (N0142) 1:5,000
Substance P/TAC1 Rabbit Human TAC1-GST fusion protein (Ag4790) Proteintech (13839-1-AP) 1:200
β-actin Mouse Monoclonal, clone AC-15 Sigma Aldrich (A1978) 1:5,000
Secondary antibody Life technologies, Invitrogen
Alexa 488 Goat anti-mouse A-11001 1:2,000
Alexa 488 Goat anti-guinea pig A-11073 1:2,000
Alexa 568 Goat anti-mouse A-11004 1:2,000
Alexa 647 Goat anti-mouse A-21235 1:2,000
Alexa 568 Goat anti-rabbit A-11011 1:2,000
Alexa 488 Goat anti-rabbit A-11008 1:2,000
Alexa 647 Goat anti-rabbit A-21244 1:2,000
Fab fragments Goat anti-mouse (IgG H&L) Rockland inc. (810–1102) 1:50
STAR Red Goat anti-rabbit (IgG) Abberior (2-0012-011-9) 1:100