Table 1.
Comparative abundance of transcripts encoding flavonoid and phenylpropanoid biosynthetic genes between skin and flesh of blueberry fruit.
| Gene model | Average flesh | Average skin | Log2fold | p-value | Best Arabidpsis blast match (or other if poor match) |
|---|---|---|---|---|---|
| 36273 | 0.3 | 294.0 | 7.72 | 1.99E-30 | Camellia sinensis, flavonoid 3′,5′-hydroxylase (F3′,5′H). (AT5G06900) |
| 38016 | 286.3 | 18289.0 | 6.15 | 0 | AT4G22880.1, ANTHOCYANIDIN SYNTHASE (ANS, LDOX) |
| 39197 | 1247.0 | 71130.7 | 5.99 | 0 | AT4G22880.1, LDOX, TT18 |
| 7214 | 14.7 | 944.3 | 5.98 | 7.15E-75 | Populus trichocarpa, F3′,5′H (AT5G07990.1, TT7) |
| 39241 | 67.0 | 3587.0 | 5.87 | 0 | AT5G13930.1, chalcone synthase (CHS, TT4) |
| 24097 | 81.0 | 4548.7 | 5.80 | 7.97E-66 | AT2G37040.1, phenylalanine ammonia-lyase (AtPAL1) |
| 25738 | 370.7 | 17356.7 | 5.70 | 0 | AT5G13930.1, CHS |
| 32663 | 68.3 | 3082.0 | 5.62 | 3.01E-271 | AT4G22880.1, LDOX |
| 36277 | 0.3 | 66.3 | 5.38 | 2.26E-11 | Populus trichocarpa, F3′,5′H (AT5G07990.1) |
| 3392 | 74.3 | 3274.0 | 5.37 | 2.46E-37 | AT2G37040.1, PAL |
| 13816 | 247.7 | 6724.0 | 4.91 | 0 | AT2G37040.1, AtPAL1 |
| 8284 | 282.0 | 7233.0 | 4.83 | 0 | AT3G53260.1, AtPAL2 |
| 32449 | 1.7 | 82.0 | 4.73 | 6.59E-11 | AT2G30490.1, cinnamate 4-hydroxylase (C4H) |
| 7478 | 1541.7 | 28419.7 | 4.36 | 0 | Vitis vinifera F3′H (AT5G07990.1, TT7) |
| 7549 | 3637.3 | 61963.3 | 4.25 | 0 | AT5G13930.1, CHS (TT4) |
| 32451 | 693.7 | 11460.3 | 4.20 | 0 | AT2G30490.1, C4H |
| 36275 | 0.7 | 34.7 | 3.98 | 0.00000429 | Vitis vinifera F3′,5′H-2-like, (AT5G07990) |
| 24099 | 6.7 | 123.7 | 3.96 | 1.76E-12 | AT3G10340.1, AtPAL4 |
| 33001 | 51.0 | 649.3 | 3.77 | 2.78E-46 | AT3G51240.1, flavanone 3-hydroxylase (F3H) |
| 6667 | 2434.7 | 23754.0 | 3.45 | 0 | AT3G51240.1, F3H |
| 34918 | 5.3 | 79.0 | 3.40 | 0.00000157 | AT3G63170.1, chalcone isomerase (CHI) |
| 1248 | 1788.0 | 15398.7 | 3.27 | 0 | AT5G42800.1, dihydroflavonol 4-reductase (DFR, TT3) |
| 17397 | 295.7 | 2543.7 | 3.24 | 1.2E-92 | AT5G13930.1, CHS (TT4) |
| 32630 | 1030.7 | 8589.3 | 3.21 | 5.37E-217 | AT5G42800.1, DFR (TT3, M318) |
| 842 | 1363.7 | 10223.3 | 3.06 | 3.86E-192 | AT5G13930.1, CHS (TT4) |
| 30312 | 2316.3 | 16659.7 | 3.00 | 8.55E-284 | AT3G21240.1, coumarate:CoA ligase 2 (4CL2, AT4CL2) |
| 18037 | 894.3 | 6141.0 | 2.93 | 2.83E-96 | AT5G13930.1, CHS (TT4) |
| 32793 | 22.7 | 158.0 | 2.67 | 0.00000479 | AT5G08640.1, flavonol synthase 1 (FLS, ATFLS1) |
| 592 | 2.0 | 26.0 | 2.62 | 0.003348852 | AT1G61720.1, NAD(P)-binding Rossmann-fold superfamily (BAN) |
| 15090 | 370.7 | 1710.3 | 2.35 | 1.95E-46 | AT5G17050.1, UDP-glucose: flavonoid 3-O-glucosyltransferase |
| 33905 | 331.0 | 47.0 | –2.58 | 9.7E-16 | AT5G54160.1, flavonol 3-O-methyltransferase |
RNA samples were compared using RNA-seq. Thirty candidate flavonoid biosynthetic genes had higher transcript abundance in the skin sample (>2 log2fold change), while one had higher transcript abundance in the flesh sample (>-2 log2fold change). Gene model number is abbreviated with full number shown in Supplementary Table S2. Data shown are genes with differential expression with a cut off value of greater than 2 log2fold.