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. 2018 Sep 15;20(10):1083–1093. doi: 10.1016/j.neo.2018.08.010

Figure 3.

Figure 3

Estrogen induces claudin-1 expression in cervical adenocarcinoma cells. (A) CLDN1 expression was induced by E2 treatment (24 hours) in CAC-1, Hela229, and HCA1 cells. (B) Immunofluorescence of CLDN1 (green) was induced by E2 treatment (10 nM, 24 hours) in HCA1 and Hela229 cells. (C) Expression profile of GPR30 (membrane-bound estrogen receptor) and ERα (classical estrogen receptor) in cervical adenocarcinoma cells. MCF7 cells (breast cancer cells) were used as a positive control for ERα expression. Western blotting. (D) Immunofluorescence of GPR30 (green) in cervical adenocarcinoma cells. (E) CLDN1 expression was induced by G1 (GPR30 selective agonist), but not by PPT (ERα selective agonist) or DPN (ERβ selective agonist), treatment (10 nM, 24 hours) in cervical adenocarcinoma cells. The relative intensity of each band is shown over each immunoblot after normalization for the levels of actin. CLDN1: claudin-1.