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. 2018 Sep 15;20(10):1083–1093. doi: 10.1016/j.neo.2018.08.010

Figure 4.

Figure 4

GPR30 mediates induction of claudin-1 expression in cervical adenocarcinoma cells. (A) CLDN1 expression was induced by G1 treatment (24 hours) in CAC-1, Hela229, and HCA1 cells. (B) Immunofluorescence of CLDN1 was induced by G1 treatment (10 nM, 24 hours) in Hela229 and HCA1 cells. (C) G1-induced CLDN1 expression was inhibited by pretreatment with G15 (GPR30 selective antagonist) for 24 hours in CAC-1 and Hela229 cells. (D) GPR30 expression was knocked down by GPR30-specific siRNA in HCA1 cells. (E) G1-induced CLDN1 expression was inhibited by knockdown of GPR30 expression with GRP30-specific siRNA in HCA1 cells. (F) G1 treatment (10 nM) increased phosphorylation of ERK and Akt in Hela229 cells. (G) G1-induced CLDN1 expression was inhibited by pretreatment with U0126 (10 μM) and LY294002 (10 μM) in CAC-1 and Hela229 cells. (A, C-G) Western blotting and (B) confocal microscopy. The relative intensity of each band is shown over each immunoblot after normalization for the levels of actin. CLDN1: claudin-1.