Figure 3.

The Insertion of the IS2 Element into the LV Backbone Reduces Their Efficiency to Generate Vector Genomes in Target Cells

(A) Representative plots showing eGFP expression profiles of 293T cells transduced with integrative LVs (left plots) and IDLVs (right plots) with (SE-IS2) or without (SE) the IS2 element. All the experiments were carried out using 0.7 viral particle/cells. (B) Graph showing the amounts of reverse-transcribed products (vector genomes) in 293T cells transduced with LVs (left bars) and IDLVs (right bars) with (SE-IS2) or without (SE) the IS2 element at 72 hr after transduction. Values represent means ± SEM of at least four separate experiments (*p < 0.05).