Figure 6.

The IS2 Sequence Improves Transgene Expression in Neural Progenitor Cells and Differentiated Neuron-like Cells

(A) Representative image (left) and plots (right) showing eGFP expression profiles of undifferentiated neural stem cells transduced at MOI = 3 with SE, SE-IS2, SEWP, and SEWP-IS2-IDLVs and analyzed 72 hr post-transduction. The percentage (%) of the eGFP⁺ cells and the eGFP expression levels (MFI) are shown in each plot. (B) Representative image (left) and plots (right) showing eGFP expression profiles of differentiated neuron-like cells (positive for β-tubulin, see Materials and Methods for details) transduced at MOI = 3 with SE-, SE-IS2-, SEWP-, and SEWP-IS2-IDLVs and analyzed 72 hr post-transduction. The percentage (%) of the eGFP⁺ cells and the eGFP expression levels (MFI) are shown in each plot. (C) Graph showing the relative expression levels of eGFP (MFI) in NPCs transduced with the different IDLVs and normalized to the levels observed in SE-IDLVs. (D) Graph showing the relative expression levels of eGFP (MFI) in differentiated NPCs (neuron-like cells) transduced with the different IDLVs and normalized to the levels observed in SE-IDLVs. Values represent means ± SEM of at least four separate experiments (*p < 0.05).