Figure 7.

The IS2 Sequence Improves Transgene Expression in iPSCs

(A) Representative plots showing eGFP expression profiles of iPSCs transduced with the SE, SE-IS2, SEWP, and SEWP-IS2 IDLVs at MOI = 10. The percentage (%) of the eGFP⁺ population and the relative transgene expression levels (MFI) are shown in each plot. (B) Graphs showing the relative percentage of eGFP⁺ cells (top) and the relative transgene expression levels (bottom) in iPSCs transduced with different IDLVs and normalized to the levels obtained with SE-IDLVs. The values represent means ± SEM of at least four separate experiments (*p < 0.05).