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. 2018 Aug 18;13:16–28. doi: 10.1016/j.omtn.2018.08.007

Table 1.

Pairs of Primers Used to Establish the 2-LTR Relative to the Total IDLV DNA Ratio by Real-Time PCR and Estimate the cDNA Amount of eGFP

Primer Pair Orientation Sequence Amplified Forms
q2-LTR Fw 5′-GCCTCAATAAAGCTTGCCTTG-3′ 2-LTR circles
Rv 5′-TGGGAGTGAATTAGCCCTTCCA-3′
ΔU3 Fw 5′-GATCTGCTTTTTGCTTGTACT-3′ total viral DNA
PBS Rv 5′-GAGTCCTGCGTCGAGAGAGC-3′
qhAlb Fw 5′-GCTGTCATCTCTTGTGGGCTGT-3′ genomic normalization
Rv 5′-ACTCATGGGAGCTGCTGGTTC-3′
GFP Fw 5′-GCCCGACAACCACTACCT-3′ GFP cDNA
Rv 5′-CGTCCATGCCGAGAGTGA-3′
GAPDH Fw 5′-ATGGGGAAGGTGAAGGTCG-3′ GAPDH cDNA
Rv 5′-GGGGTCATTGATGGCAACAATA-3′

The q2-LTR pair of primer flanks the 2-LTR circle junction. The second pair of primers amplify all forms of reverse transcribed products (bottom). The qhAlb is used as a control for genomic normalization. GFP cDNA primers pair amplifies the eGFP cDNA. GAPDH primers were used for cDNA normalization. FW, forward; Rv, reverse.