Table 1.

Pairs of Primers Used to Establish the 2-LTR Relative to the Total IDLV DNA Ratio by Real-Time PCR and Estimate the cDNA Amount of eGFP

Primer Pair	Orientation	Sequence	Amplified Forms
q2-LTR	Fw	5′-GCCTCAATAAAGCTTGCCTTG-3′	2-LTR circles
	Rv	5′-TGGGAGTGAATTAGCCCTTCCA-3′
ΔU3	Fw	5′-GATCTGCTTTTTGCTTGTACT-3′	total viral DNA
PBS	Rv	5′-GAGTCCTGCGTCGAGAGAGC-3′
qhAlb	Fw	5′-GCTGTCATCTCTTGTGGGCTGT-3′	genomic normalization
	Rv	5′-ACTCATGGGAGCTGCTGGTTC-3′
GFP	Fw	5′-GCCCGACAACCACTACCT-3′	GFP cDNA
	Rv	5′-CGTCCATGCCGAGAGTGA-3′
GAPDH	Fw	5′-ATGGGGAAGGTGAAGGTCG-3′	GAPDH cDNA
	Rv	5′-GGGGTCATTGATGGCAACAATA-3′

The q2-LTR pair of primer flanks the 2-LTR circle junction. The second pair of primers amplify all forms of reverse transcribed products (bottom). The qhAlb is used as a control for genomic normalization. GFP cDNA primers pair amplifies the eGFP cDNA. GAPDH primers were used for cDNA normalization. FW, forward; Rv, reverse.