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. 2018 Sep 11;9:2054. doi: 10.3389/fimmu.2018.02054

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Copyright © 2018 Baliu-Piqué, Verheij, Drylewicz, Ravesloot, de Boer, Koets, Tesselaar and Borghans.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of deuterium enrichment and summary of the estimated lifespan of CD4⁺ and CD8⁺ memory T-cells from blood, BM and LN. (A) Best fits to the level of deuterium enrichment measured in the DNA of CD4⁺ and CD8⁺ memory (CCR7⁻) T-cells from blood, BM and LN. Label enrichment in the DNA was scaled between 0 and 100% by normalizing to the maximum enrichment in granulocytes (See material and methods). (B) Estimated lifespans of CD4⁺ and CD8⁺ memory T-cells in days, and their respective 95% confidence limits. (C) Correlation between deuterium enrichment in BM and blood, and LN and blood. The gray dashed line represents the X = Y line.