Table 1.
Immunophenotypic characterization of BM-derived T-cells.
| Blood (N = 18) | BM (N = 20) | LN (N = 18) | ||||
|---|---|---|---|---|---|---|
| Lymphocytes | 59.0 (17.6) | 23.6 (6.7) | 69.9 (12.7) | |||
| CD4+ | 18.2 (7.0) | 1.4 (1.9) | 31.9 (12.2) | |||
| CD8+ | 24.4 (8.1) | 6.7 (6.3) | 16.4 (4.6) | |||
| CD4/CD8 ratio | 0.9 (0.6) | 0.2 (0.1) | 2.1 (1.0) | |||
| CD4+ | CD8+ | CD4+ | CD8+ | CD4+ | CD8+ | |
| CCR7− (memory) | 40.0 (17.5) | 80.2 (13.3) | 88.3 (5.7) | 97.8 (1.3) | 30.2 (7.7) | 46.0 (16.1) |
| CD62L− | 45.5 (17.1) | 64.6 (14.8) | 92.7 (4.3) | 94.0 (2.6) | 41.6 (14.8) | 43.0 (13.7) |
| CCR7−CD62L− (DN-M) | 26.5 (9.0) | 59.8 (13.1) | 83.4 (6.7) | 92.3 (2.6) | 20.7 (6.9) | 32.6 (14.0) |
Flow cytometric analysis was used to assess the percentage of blood, LN and BM-derived mononuclear cells expressing CD4, CD8, CCR7, and CD62L. Percentage lymphocytes based on FSC/SSC within the total events, percentage of CD4+ and CD8+ cells within total lymphocytes, CD4/CD8 ratios, and percentage of CCR7− (memory), CD62L− and CCR7−CD62L− (DN-M) cells within the total CD4+ and CD8+ lymphocyte populations are shown. N = number of animals analyzed. We report mean and standard deviation (SD).