Table 3.

Specific primer information for tea plant necrotic ring blotch virus (TPNRBV) and tea plant line pattern virus (TPLPV) detection.

Virus segment	Primer name	Sequence (5′ to 3′)	Annealing temperature	Amplicon length
TPNRBV RNA1	TPNRBV1-F	GCCCTGACAACGCAAAAGAACTGATG	52°C	535 bp
	TPNRBV1-R	GTGACGGGATATTTTTGGACGACTGT
TPNRBV RNA2	TPNRBV2-F	GGGCCGGGGTGTGGAAAAACTT	52°C	551 bp
	TPNRBV2-R	TTCTTATCATCCCGGCAAAACACA
TPNRBV RNA3	TPNRBV3-F	TTCGCCACTCACAAAGACAACAAACT	52°C	459 bp
	TPNRBV3-R	GTAGCGGAGCGGAAAGAAAAGACT
TPNRBV RNA4	TPNRBV4-F	TCAGTGGCGCGATTATCAGAAGGTA	52°C	407 bp
	TPNRBV4-R	CGCGCAAGAAGTCGGTCAAAAC
TPLPV RNA1	TPLPV1-F	AAGGTGGCGAGGTCAGTTTCAGTTCA	52°C	513 bp
	TPLPV1-R	TCCCCATAGGTTCATCTTGTAGCAGTCG
TPLPV RNA2	TPLPV2-F	CCTATGGAGCTCTATGACGCAAATGAT	52°C	403 bp
	TPLPV2-R	TCGACAGAGAAGTGATGGGGAAATAC
TPLPV RNA3	TPLPV3-F	TCAGATGCGACCAATGGAACTCAACT	52°C	421 bp
	TPLPV3-R	CCCGCGCTAATCCTCTCAAGACTAACTA