Table 4.

Detection of TPNRBV via reverse-transcription (RT)-PCR in different tissues of tea plant displaying symptoms of tea plant necrotic ring blotch disease.

Number of biological replicationsb	Different tissuesa
	Apical buds	Young stems	Upper leaves	Lower leaves	Roots
1	+ + + +	+ + + +	+ + + +	+ + + +	+ + + +
2	+ + + +	+ + + +	+ + + +	+ + + +	+ + + +
3	+ + + +	+ + + +	+ + + +	+ + + +	+ + + +
4	+ + + +	+ + + +	+ + + +	+ + + +	+ + + +
5	+ + + +	+ + + +	+ + + +	+ + + +	+ + + +

^aSymbols (+ + + +) indicate positive detection by RT-PCR for the four segments (RNA1, RNA2, RNA3, and RNA4) of TPNRBV. ^bTissues collected from different TPNRBV diseased tea plants were used as one biological replication.