Table 5.

Detection of TPNRBV via reverse-transcription (RT)-PCR in individual shoots or leaves of tea plant showing mixed symptoms of tea plant necrotic ring blotch disease.

Number of biological replicationsb	Shootsa		Leavesa
	Upper leaves without symptoms	Lower leaves with symptoms	Leaf half with symptoms	Leaf half without symptoms
1	+ + + +	+ + + +	+ + + +	+ + + +
2	+ + + +	+ + + +	+ + + +	+ + + +
3	+ + + +	+ + + +	+ + + +	+ + + +
4	+ + + +	+ + + +	+ + + +	+ + + +
5	+ + + +	+ + + +	+ + + +	+ + + +
6	+ + + +	+ + + +	+ + + +	+ + + +
7	+ + + +	+ + + +	+ + + +	+ + + +
8	+ + + +	+ + + +	+ + + +	+ + + +
9	+ + + +	+ + + +	+ + + +	+ + + +
10	+ + + +	+ + + +	+ + + +	+ + + +
11	+ + + +	+ + + +	+ + + +	+ + + +
12	+ + + +	+ + + +	+ + + +	+ + + +
13	+ + + +	+ + + +	+ + + +	+ + + +
14	+ + + +	+ + + +	+ + + +	+ + + +
15	+ + + +	+ + + +	+ + + +	+ + + +

^aSymbols (++++) indicate positive detection by RT-PCR for the four segments (RNA1, RNA2, RNA3, and RNA4) of TPNRBV. ^bIndividual shoot/leaf from different TPNRBV diseased tea plant was used as one biological replication.