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. 2018 Aug 18;13:29–43. doi: 10.1016/j.omtn.2018.08.006

Figure 7.

Figure 7

miR-26b Mimic Preserves the Native Microvasculature Network Morphology following Ischemia Injury

Representative confocal microscopy images of thick longitudinal sections for the ischemic adductor muscles injected with control mimic (left panels) or miR-26b mimic (right panels) at day 3 after ischemia. Upper panel: effect of exogenous miR-26b mimic injection on the survival of native muscle fibers and microvasculature network is shown. Asterisks denote the necrotic (dead) muscle fibers. Dotted line delimits the border of necrotic area. Myocytes (phalloidin, green), microvessels (isolectinB4, red), and nuclei (DAPI, blue) are shown. Scale bar, 340 μm. Middle panel: effect of exogenous miR-26b mimic injection on the native microvasculature network and the infiltration of leukocytes in the ischemic muscles is shown. Leukocytes (CD45, green) and microvessels (isolectinB4, red) are shown. Scale bar, 340 μm. Lower panel: inset of a higher magnification shows the microvascular network architecture in the necrotic area of ischemic adductor muscle. Arrowheads point to the preserved native microvasculature network in the necrotic areas. Leukocytes (CD45, green), microvessels (isolectinB4, red), and nuclei (DAPI, blue) are shown. Scale bar, 80 μm.