Figure 1.

HIV DNA observed in CD4 T cells interacting with autologous CD8 T cells throughout the course of HIV infection. Following incubation of CD4 T cells with autologous CD8 T cells in situ PCR of HIV LTR DNA was performed, followed by hybridization with a 56nt long FITC-labeled DNA probe (green). Anti-perforin antibody labeled by Alexa Fluor 647 (red) was used for immunohistochemistry (A, B). The slides were then observed by confocal microscopy (x63). CD4 T cells were conjugated by autologous CD8 T cells, as observed in: (A) acute HIV infection, (B) chronic HIV infection, (C) a chronic HIV-infected patient on ART, (D,E) an AIDS patient, (F) CD4 T cells from an AIDS patient incubated without CD8 T cells, (G) a healthy control.