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. 2018 Sep 11;9:2068. doi: 10.3389/fimmu.2018.02068

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Copyright © 2018 Sevilya, Chorin, Gal-Garber, Zelinger, Turner, Avidor, Berke and Hassin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Conjugation and apoptosis of CD4 T cells by autologous CD8 T cells procured from patients on ART and analyzed by the ImageStream. (A) Apoptosis assay of CD4 T cells with CD8 T cells procured from a 43-year-old female patient on ART with undetectable viral load and a CD4 T cell count of 507 cells/mm³ (39%). The CD4 T cells in apoptosis are positive for activated caspase 3 (orange) and DNA fragmentation, TUNEL assay (yellow), CD8 T cells (red), DAPI (purple). (B) Quantitative analysis of conjugation and apoptosis of CD4 and CD8 T cells in five patients on ART compared to five healthy controls. ** indicate P-values <0.01, two-tailed paired t-test.