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. 2018 Sep 11;9:2080. doi: 10.3389/fimmu.2018.02080

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Copyright © 2018 Li, Sheng, Liu, Wang, Zhao, Yang, Jia, Li, Wang, Xie, Xu, Sun, Qiu and Song.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of an effective HBV-specific CRISPR/SaCas9 system. (A) Illustration of gRNA-targeted sequences located in the HBV genome. (B) Results of HBsAg measurement in cell-culture supernatants on the 3rd day after co-transfection with CRISPR-SaCas9 and pGL3-HBV1.2 expression vector in 293T cells. Error bars, SD; n = 3. (C) CCK-8 assay performed at the indicated time points after transfection (absorbance at 450 nm, A450). (D) Level of HBeAg in cell-culture supernatants on the 3rd day after co-transfection with CRISPR-SaCas9 and pGL3-HBV1.2 in 293T cells. Error bars, SD; n = 3.