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. 2018 Sep 11;9:2080. doi: 10.3389/fimmu.2018.02080

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Copyright © 2018 Li, Sheng, Liu, Wang, Zhao, Yang, Jia, Li, Wang, Xie, Xu, Sun, Qiu and Song.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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rAAV8-mediated CRISPR-SaCas9 introduction disrupted HBV DNA in the liver of HBV-Tg mice. (A) Assessment of off-target cutting by gRNA-Sa4. The results of the surveyor assay in liver cells treated with gRNA-empty (–) and gRNA-Sa4 (+) are shown; gRNA-Sa4 and gRNA-Sa4 off-target sites 1, 2, 3, 4, and 5 were PCR-amplified. T7EI nuclease-cleaved gRNA PCR products are indicated by Red arrow heads. (B) Representative views of target S4 region deletion in gRNA-S4-treated mice, generated using Integrative Genomics Viewer. Black bars: deletions; gray bars: sequencing reads.