Fig. 6.

p53 upregulation and apoptosis predominantly occur in either cells in G1 or postmitotic cells. (A) IF for γH2AX (green) and EdU (magenta) in sagittal sections of E12 mutant (mut) pallium, with pie charts showing the percentage of EdU⁺ cells of the nonpyknotic (non-pyk.) γH2AX⁺ and DAPI⁺ populations. See also Fig. S5. (B) IF for p53 (green) and EdU (magenta) in sagittal sections of E12 mut pallium, with pie charts showing the percentage of EdU⁺ cells of the p53⁺ and DAPI⁺ populations. (C) IF for Aurora B (green, AurB) and p53 (magenta) in sagittal sections of the E12 mut pallium, with pie charts showing the percentage of G2 Aurora B⁺ cells of the p53⁺ and DAPI⁺ populations (left), and the percentage of mitotic (M) Aurora B⁺ cells of the p53⁺ and DAPI⁺ populations (right). (D) IF for CC3 (green), EdU (magenta) and TUNEL (yellow) in sagittal sections of E12 mut pallium, with pie charts showing the percentage of EdU⁺ cells of the nonpyknotic CC3⁺ cell population. (E) IF for CC3 (green), Aurora B (magenta) and TUNEL (yellow) in sagittal sections of the E12 mut pallium, with quantification. Scale bars: 30 µm. Quantification data are presented as mean±s.e.m. For details of statistical methods, see Table S8. n.s., not significant, ***P<0.001.