FIGURE 2.

Functional validation of the signal peptide of Pst_8713. The sequence of the putative Pst_8713 signal peptide was fused in-frame to the invertase sequence in the pSUC2 vector and transformed into yeast strain YTK12. The untransformed YTK12 strain, empty pSUC2 vector and the first 25 amino acids of non-secreted Mg87 protein from Magnaporthe oryzae were used as negative controls, and the oomycete effector Avr1b from Phytophthora sojae was used as a positive control. Only yeast strains that are able to secrete invertase can grow on YPRAA media.