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. 2018 Sep 11;9:1294. doi: 10.3389/fpls.2018.01294

FIGURE 6.

FIGURE 6

Overexpression of Pst_8713 in N. benthamiana suppressed expression of PTI-related marker genes. (A) Callose deposition in leaves of N. benthamiana inoculated with EtHAn, pEDV6:dsRED, and pEDV6:Pst_8713 stained with aniline blue. Bar = 200 μm. (B) The average number of callose deposits in tobacco leaves expressed with pEDV6:dsRED or pEDV6:Pst_8713. Bars indicate means ± SE of three independent biological replicates with 30 unit areas per replicate. The different letters indicate significant differences (P < 0.05) according to ANOVA. (C) Bacterial growth in EtHAn, pEDV6:dsRED, or pEDV6:Pst_8713 infiltrated tobacco leaves. Numbers of bacteria were evaluated at 0 and 24 hpi. Each time-point is average of three biological replicates. Bacteria in tobacco inoculating EthAn carrying pEDV6:Pst_8713 grew ∼2.2-fold better than EthAn carrying pEDV6:dsRED at 24 hpi. (D) Transcription level fold changes of plant defense-related genes PR1a, PR2, and WRKY12 in leaves of N. benthamiana transformed with pEDV6: dsRED or pEDV6: Pst_8713 at 24 hpi with EtHAn. NbActin was used as the internal reference gene. Bars indicate means of three independent biological replicates (±SE) and different letters indicate significant differences (P < 0.05) according to ANOVA.