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. 2018 Sep 11;9:1899. doi: 10.3389/fmicb.2018.01899

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Copyright © 2018 Cao, Tan, Zhang, Han, Hou, Ma and Cai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NagR_Bt-binding sites are detected by EMSAs. (I) Genes upregulated in the ΔnagR mutant compared to wild-type strain Bti75; (II) Genes downregulated in the ΔnagR mutant compared to wild-type strain Bti75; (III) Genes with recognized dre sites but not differentially expressed in the ΔnagR mutant compared to wild-type strain Bti75. (a): Each 0.2 μM 40 bp-Pdre was electrophoresed after incubation with various concentrations of NagR_Bt. (b): EMSAs to determine the specific binding of NagR_Bt. Lane 1, Pdre (Bio), NagR_Bt, and 0.5 μg/μL salmon sperm DNA for non-specific competitor (N); Lane 2, Pdre (Bio), NagR_Bt; Lane 3, Pdre (Bio); Lane 4, Pdre (Bio), NagR_Bt, and a 400-fold excess of unlabeled Pdre for specific competitor (S). All the non-specific and specific competition assays used 3.0 μM NagR_Bt and 0.2 μM Pdre (Bio). Different letters represent different genes. A: nagP, BTF1_00220; B: outer surface protein, BTF1_01610; C: lpmo, BTF1_11665; D: chiA, BTF1_16665; E: ptsH, BTF1_18560; F: nagA, BTF1_18590; G: hypothetical protein, BTF1_19070; H: crr, BTF1_24980; I: hypothetical protein, BTF1_19025; J: pgi, BTF1_23020; K: lytR family protein, BTF1_00745; L: peptidase, BTF1_01335; M: major facilitator superfamily protein, BTF1_02575; N: TetR family transcriptional regulator, BTF1_03055; O: pepT, BTF1_19055; P: wall-associated protein, BTF1_23845; Q: chbB, BTF1_24430; R: bofA, BTF1_26160.