Table 1.
Differential activation, accumulation and influence of NKT cell subsets on other innate cells in liver inflammation.
| iNKT cellsa | Type II NKT cellsb | Myeloid cellsc | NK cellsd | ||||
|---|---|---|---|---|---|---|---|
| Liver | Tetramer+ | Cytokinese | FasL | Apoptosisf | |||
| IRI | NC | IFNγ | NC | NC | ND | ↑↑ | ↑ |
| Con A | ↓ | IFNγ, IL-4 | ↑↑ | ↑↑ | ND | ↑↑ | ↑ |
| Alcohol C + B | ↑↑ | IFNγ | NC | NC | NC | ↑↑ | NC |
| Alcohol Chronic | NC/↓ | IFNγ | ↑ | ↑ | ↑↑ | ↑↑ | NC |
| CDAA diet | ↓ | IL-17, IFNγ, IL-4, IL-13 | ND | ND | ND | ↑↑ | ↑ |
iNKT cells were analyzed in liver mononuclear cells using αGalCer-loaded CD1d tetramers and identified as double positive cells for αGalCer/CD1d tetramer and TCRβ (αGalCer/CD1d tetramer+TCRβ+) in the NK1.1+ gate.
Type II NKT cells were also analyzed in liver mononuclear cells and defined as double positive cells for TCRβ and NK1.1 (TCRβ+NK1.1+) in the αGalCer/CD1d tetramer− gate or sulfatide/CD1d-tetramer+ cells.
Myeloid cells included both monocytes, neutrophils and macrophages.
NK cells were defined as NK1.1+TCRβ− cells.
Cytokines secretion was determined in culture supernatants after stimulation with PMA & Ionomycin by FACS using BD Cytometric bead array.
Apoptotic cells were identified as Annexin V+ cells.
IRI, Ischemia Reperfusion Injury; Con A, Concanavalin A-induced hepatitis; Alcohol C+B, chronic feeding of Lieber-DeCarli liquid diet for 10 days plus binge on day 11; Alcohol Chronic, chronic feeding of Liber deCarli liquid alcohol diet for 6–8 weeks; CDAA diet, chronic feeding of Choline deficient amino acid defined diet for 20 weeks. NC, not changed; ND, not determined.