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. 2018 Sep 11;9:1299. doi: 10.3389/fpls.2018.01299

Table 2.

Synthesis of all protein pair combinations performed in the BiFC hetero-dimerization assay.

Heterodimers p29-p15 p29-MP p29-p24 p15-MP p15-p24 MP-p24
ORF-NYFP + CYFP-ORF +++ +
ORF-NYFP + ORF-CYFP +++ + ++
NYFP-ORF + CYFP-ORF +
NYFP-ORF + ORF-CYFP
ORF-CYFP + NYFP-ORF +++ + ++
ORF-CYFP + ORF-NYFP +++ +++ +++
CYFP-ORF + NYFP-ORF ++ +
CYFP-ORF + ORF-NYFP +++ ++ +
Negative controls p29 p15 MP p24
CYFP-ORF+NYFP-Cyt
ORF-CYFP+NYFP-Cyt
NYFP-ORF+CYFP-ER

The symbols –, +, ++, and +++ correspond to the absence, low, medium or high fluorescence signals, respectively. NtYFP and CtYFP correspond to half N- and C-terminal of the YFP protein, respectively.