Table 3.
Synthesis of all protein pair combinations performed in the BiFC topology assay for p29, p15, MP, and p24 CiLV-C proteins.
| Membrane topology | NYFP-Cyt | CYFP-Cyt | NYFP-ER | CYFP-ER |
|---|---|---|---|---|
| NtYFP-ER | – | +++ | ||
| NtYFP-Cyt | +++ | – | ||
| p29-NtYFP | +++ | – | ||
| p29-CtYFP | – | – | ||
| NtYFP-p29 | +++ | – | ||
| CtYFP-p29 | – | – | ||
| p15-NtYFP | +++ | – | ||
| p15-CtYFP | – | – | ||
| NtYFP-p15 | +++ | – | ||
| CtYFP-p15 | – | – | ||
| MP-NtYFP | +++ | – | ||
| MP-CtYFP | – | – | ||
| NtYFP-MP | – | – | ||
| CtYFP-MP | – | – | ||
| p24-NtYFP | – | +++ | ||
| p24-CtYFP | – | – | ||
| NtYFP-p24 | +++ | – | ||
| CtYFP-p24 | – | – |
The symbols –, +, ++, and +++ correspond to the absence, low, medium or high fluorescence signals, respectively. NYFP and CYFP correspond to half N- and C-terminal of the YFP protein, respectively.