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. 2018 Sep 11;9:1325. doi: 10.3389/fpls.2018.01325

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Copyright © 2018 García, Corpas, Lucena, Alcántara, Pérez-Vicente, Zamarreño, Bacaicoa, García-Mina, Bauer and Romera.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of Fe deficiency and foliar application of Fe on the expression of (A) Fe acquisition genes FRO2, IRT1, and FIT; and (B) ethylene synthesis genes SAM1, ACS6 and ACO2, in roots of Arabidopsis WT Columbia plants. Plants were grown in nutrient solution with Fe (+Fe). Some of them were transferred to nutrient solution without Fe 3 days before harvest (–Fe). Half of the –Fe plants were sprayed with Fe 24 h before harvest (–Fe+fol Fe). Relative expression was calculated in relation to +Fe. Data represent the mean ± SE of three independent biological replicates. Within each gene, bars with different letters indicate significant differences (P < 0.05).