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. 2018 Sep 11;9:1325. doi: 10.3389/fpls.2018.01325

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Copyright © 2018 García, Corpas, Lucena, Alcántara, Pérez-Vicente, Zamarreño, Bacaicoa, García-Mina, Bauer and Romera.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Time course experiment of the effect of Fe deficiency on the expression of (A) GSNOR1, Fe acquisition genes (B) FIT, (C) bHLH38, (D) IRT1 and (E) FRO2), and on (F) ferric reductase activity, in roots of Arabidopsis WT Columbia plants. Plants were grown in nutrient solution with Fe (+Fe). Some of them were transferred to nutrient solution without Fe (–Fe) 6, 12, 24, 48, or 72 h before determination of ferric reductase activity or harvest. For genes, data represent the mean ± SE of three independent biological replicates. Relative expression was calculated in relation to +Fe. For ferric reductase activity, data represent the mean ± SE of six replicates.