Table I.
Primer sequences with annealing temperature and restriction enzymes used for genotyping.
| Single nucleotide polymorphism | Primer sequence, 5′-3′ | Enzyme for RFLP | Product size, bp |
|---|---|---|---|
| Vitamin D receptor gene | |||
| rs731236 | F: TGGTGGGATTGAGCAGTGAG | TaqI | Uncut: 249 |
| Cut: 101/148 | |||
| R: GTACTGCTTGGAGTGCTCCT | |||
| rs1544410 | F: AACCTGAAGGGAGACGTAGCA | BsmI | Uncut: 283 |
| Cut: 200/83 | |||
| R: TTGTACCCTGCCCGCAAGAAA | |||
| rs2228570 | F: ACCAAGGATGCCAGCTGG | FokI | Uncut: 266 |
| Cut: 19/63/184 | |||
| R: GCTTCTTCTCCCTCCCTTTC | |||
| Tumor necrosis factor superfamily member 11 gene | |||
| rs9533155 | F: GCCACAGTTCTGAATAGAGG | BsaJI | Uncut: 498 |
| Cut: 123/375 | |||
| R: GGATAAGGATTGCACCTCAG | |||
| rs9533156 | F: GCCACAGTTCTGAATAGAGG | TspRI | Uncut: 498 |
| Cut: 176/322 | |||
| R: GGATAAGGATTGCACCTCAG | |||
| rs9525641 | F: ATCCTAAGGAGGAAACCGAGAC | MseI | Uncut: 146 |
| Cut: 124/22 | |||
| R: GGAGGTCCAAGAGATGGGTTTA | |||