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. 2018 Sep 11;9:2086. doi: 10.3389/fimmu.2018.02086

Figure 4.

Figure 4

Positive selection at the interaction surface between Hb and N. meningitidis HpuA/HpuB. (A) omegaMap results: the posterior probability of positive selection (ω>1) along N. meningitidis HpuA and HpuB coding sequences are plotted. The hatched red lines correspond to a posterior probability of selection equal to 0.95. Positively selected codons are in red. Codon positions refer to N. meningitidis FAM18 strain (HpuA ID:A1KW50; HpuB ID: A1KW51). (B) The 3D structure of human Hb in complex with N. meningitidis HpuA (model, cyan). Hb is color-coded as in Figure 2. HpuA loops 1 and 5, involved in Hb interaction, are in yellow. Positively selected sites are in red. Positively selected sites located at the contact interface are labeled. Hb-bound heme molecules are represented as blue sticks. (C) Model of proposed cooperation between HpuA (cyan) and HpuB (model, blue) for heme import, associated with the membrane. Positively selected sites are in red, Hb-bound heme molecules are represented as violet sticks.