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Glycosylation of oxazoline 5 with an acceptor peptide bearing GlcNAc residue 3 catalyzed by recombinant endo-M N175Q monitored by size-exclusion HPLC on Inertsil Diol detected by UV (A), TIC of a range of m/z 1508–1509 (B), and MALDI-TOFMS of the product eluted at 10.4 min (C).
