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RAS-GTP and corresponding pERK1/2T202/Y204 levels from HeLa cells that were treated for 30 min with up to 100 μM of compound 22, 30, 32, or 34. EGF treatment (50 ng/mL for 5 min) was used as a positive control for pathway activation. Quantification values for RAS-GTP and pERK1/2T202/Y204 levels are displayed under the respective blots for each compound and are also provided in Table S1. Data are representative of three independent experiments.
