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. 2018 Jun 18;115(27):E6162–E6171. doi: 10.1073/pnas.1801909115

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Fig. 3. — Asf1a KO affects dynamic changes of histone modifications at lineage-specific genes during differentiation. (A and B) Analysis of H3K4me3 (A) and H3K27me3 (B) at the promoters of two pluripotent genes (Nanog and Oct4) by ChIP-PCR. (C and D) Analysis of H3K4me3 (C) and H3K27me3 (D) at the promoters of two lineage-specific genes (Gata4 and Gata6). Black and red bars represent ES cells and day 10 EBs, respectively. Data were from three independent experiments. Error bars represent mean ± SEM (*P < 0.05 and **P < 0.01).