Fig. 2.

Starvation-induced aay expression elevates free serine levels in heads and supports sleep suppression during starvation. (A) Schematic diagram illustrating KG05974 insertion near the 5′UTR of aay locus. (B) Western blot of head extracts in iso31 and aay^KG flies during fed and starved conditions. (C) Quantification of Western blots (n = 3) [F_(1,8) = 12.92; P = 0.0070]. (D) Serine concentrations in head extracts of iso31 control and aay^KG flies during fed and starved conditions (n = 3) [F_(1,8) = 14.53; P = 0.0052]. (E) Average sleep traces of iso31 control (black; n = 88), aay^KG/+ (light orange; n = 80), and aay^KG (orange; n = 78) flies. (F) Percentage sleep change during starvation in iso31 and aay^KG/+ controls vs. aay^KG flies. ns, P > 0.05; *P < 0.05; **P < 0.005; ***P < 0.0005; ****P < 0.0001. All error bars represent SEM.