Fig. 4.

Growth of A. baylyi strains on 1 mM guaiacol as the carbon source. Doubling times were determined for populations of ACN1686, ACN1687, and ACN1690 at the start of serial passaging. Each Gua⁺ isolate is shown adjacent to the parent from which it arose. The rightmost strain in each set is a reconstructed strain (ACN1886, ACN1739, or ACN1762) that has only the changes in catA, gcoA, and gcoB identified in an evolved isolate (ACN1850, ACN1738, and ACN1764). Reconstructed strains do not carry mutations that may have arisen in other chromosomal regions of the evolved Gua⁺ isolates.