Metabolism of guaiacol (5 mM) by P. putida strains growing on glucose. (A) In CJ514, catA and gcoA are distinct. (B) CJ516 has a catA-gcoA gene fusion analagous to that of A. baylyi ACN1738. Cultures were fed 20 mM glucose at 0, 24, 48, and 72 h. Growth was assessed by OD at 600 nm. Data points represent averages of three biological replicates, and error bars indicate the SDs.