Fig. 4.

Statistical measures obtained from the automatically determined cell positions. (A) Histogram showing the nearest-neighbor distances in the ML. The Gaussian fit reveals a mean nearest-neighbor distance of $9.7\pm 0.8$ $\mathrm{\mu }$m (95% confidence interval) with SD $\sigma =7.3\pm 0.8$ $\mathrm{\mu }$m. (B) Histogram of the nearest-neighbor distances in the GL, with a Gaussian fit indicating a mean of $4.00\pm 0.02$ $\mathrm{\mu }$m with SD $\sigma =0.45\pm 0.02$ $\mathrm{\mu }$m. (C) Histogram showing the mean gray value within the automatically detected cell volume in the ML. A Gaussian fit leads to $\left(1.17\pm 0.02\right)\cdot 10^{-3}$ with SD $\left(\sigma =2.9\pm 0.2\right)\cdot 10^{-4}$. (D) Histogram of the mean gray value in the GL. The shape is best fitted by a 2-Gaussian function with peak values of $\left(1.24\pm 0.01\right)\cdot 10^{-3}$ and $\left(1.6\pm 0.1\right)\cdot 10^{-3}$ and SDs ${\sigma }_1=\left(1.6\pm 0.1\right)\cdot 10^{-4}$ and ${\sigma }_2=\left(3.4\pm 0.1\right)\cdot 10^{-4}$ in an approximate ratio of 30:70. (E) Angular averaged pair correlation function of the cells in the GL revealing two distinct peaks at $4.16\pm 0.04$ $\mathrm{\mu }$m and $8.5\pm 0.3$ $\mathrm{\mu }$m. (F) Angular averaged structure factor of the cells in the GL. (G) Angular distribution of nearest neighbors in the GL, where $\theta \simeq 90^{○}$ corresponds to the plane in which the dendritic tree of the Purkinje cells is spreading and $\varphi =90^{○}$ is approximately parallel to the interface between the ML and GL (SI Appendix, SI Methods).