Figure 4.

Blockage of Yes‐associated protein 1 (YAP1) or phosphatase and tensin homologue (PTEN) alters the CRC cell‐mediated induction of functional myeloid‐derived suppressor cells (MDSCs) in vitro. (a) The expression of YAP1 and PTEN was knocked down in LS174T and SW620 cells by small interfering RNA (siRNA) targeting YAP1 or PTEN. (b) FACS density plots and statistical analysis showed the percentages of the MDSC population induced by SW620, LS174T, SW620‐siYAP1, LS174T‐siYAP1, SW620‐siPTEN, LS174T‐siPTEN, SW620‐siYAP1 + siPTEN or LS174T‐siYAP1 + siPTEN cell, and the CD33⁺ cells in medium alone were included as a control. Data are representative of three independent experiments. *P < 0·05 compared with the control treatment.