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. 2018 Jun 11;155(2):263–272. doi: 10.1111/imm.12949

Figure 5.

Figure 5

Blockage of Yes‐associated protein 1 (YAP1) or phosphatase and tensin homologue (PTEN) alters granulocyte–macrophage colony‐stimulating factor (GM‐CSF) secretion and is associated with the activation of P‐AKT, P‐p65 and COX‐2. (a) ELISA array for the levels of GM‐CSF, interleukin‐6 (IL‐6) and IL‐1β in the supernatants of LS174T and SW620 cell cultures with or without the presence of small interfering RNA (siRNA) ‐PTEN, siRNA‐YAP1 or siRNA‐PTEN +siRNA‐YAP1. The data are derived from three independent experiments. (b) Western blot analysis showed that P‐AKT, P‐p65, and COX‐2 expression, but not NLRP3 and IL‐1β expression, was increased with the blockage of YAP1 or PTEN. Representative data are shown; the experiment was repeated five times. (c) The signalling pathway of YAP1 and PTEN regulates tumour‐associated myeloid‐derived suppressor cell (MDSC) expansion in CRC.