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. 2018 Jun 14;155(2):238–250. doi: 10.1111/imm.12954

Figure 2.

Figure 2

Induced regulatory T (iTreg) cells suppressed cytotoxic T lymphocyte (CTL) killing redirected by ImmTAC‐NYE. (a) iTreg cells suppressed ImmTAC‐NYE‐redirected NCI‐H1299 cell lysis. Activated CD4+ T cells/iTreg cells : peripheral blood mononuclear cells (PBMC) at ratio of 1 : 2. (b) iTreg cells suppressed the ImmTAC‐NYE‐redirected T‐cell killing for T2 cells at a ratio of iTreg/activated CD4+ T cell to PBMC of 1 : 2. (c, d) CTL assays showing the killing activity redirected by titrated concentrations of the ImmTAC‐NYE with or without the presence of the iTreg cells at a PBMC to iTreg cell ratio of 2 : 1. The iTreg cells suppressed the lysis of the NCI‐H1299 cells (HLA‐A*0201, NY‐ESO‐1‐positive) (c) or T2 cells pulsed with NY‐ESO‐1157–165 peptide at a concentration of 10−7 m (d). Unpaired two‐tailed t‐test, *P < 0·05. (e) Representative flow charts showed that the iTreg cells could suppress cleaved caspase‐3 expression of the NCI‐H1299 cells during the CTL assays. The iTreg/CD4+ T cell : PBMC ratio was set as 1 : 2 (I to VI).