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. 2018 Jun 14;155(2):238–250. doi: 10.1111/imm.12954

Figure 3.

Figure 3

Mechanisms by which induced regulatory T (iTreg) cell suppressed ImmTAC‐NYE‐redirected cytotoxic T lymphocyte (CTL) killing. (a) The iTreg cells suppressed the proliferation of CD8+ T cells redirected by the ImmTAC‐NYE. A ratio of iTreg/activated CD4+ T cell : peripheral blood mononuclear cell (PBMC) of 1 : 2. The CFSE‐labelled PBMC was used as negative control. The CFSE intensity was determined after the cells were gated with anti‐CD8 antibody staining. (b, c) The PBMC was cultured with NCI‐H1299 cells at an effector to target ratio of 5 : 1, with or without the iTreg/activated CD4+ T cell at a 2 : 1 ratio of PBMC to iTreg/activated CD4+ T cell in the presence of ImmTAC‐NYE (10−9 m) over a 24‐hr period. Cultured cells were collected, after gating with anti‐CD8 antibody staining, the expression of CD25, CD107a (b), Granzyme B, Perforin (c) were determined for the CD8+ T cells. ImmTAC group: ImmTAC‐NYE + PBMC + NCI‐H1299; ImmTAC + CD4+ T‐cell group: ImmTAC‐NYE + PBMC + NCI‐H1299 + activated CD4+ T cells without induction; ImmTAC + iTreg group: ImmTAC‐NYE + PBMC + NCI‐H1299 + iTreg cells.