Table 1.
Concentration parameters of the selected plant extracts on MIA PaCa-2 cell line sensitivity depending on the extraction method
| Extraction Method | |||||||
|---|---|---|---|---|---|---|---|
| SFE | UAE | Commercial | |||||
| Concentration Parameter | RVM | OVM | |||||
| Plant extract | Ethanol | Ethanol:Water | Ethanol | Ethanol:Water | |||
| Marjoram | IC50 | > 100a | > 100a | > 100a | – | > 100 | – |
| GI50 | > 100 | 75,89 ± 2,05 | > 100 | – | > 100 | – | |
| TGI | > 100 | > 100 | > 100 | – | > 100 | – | |
| LC50 | > 100 | > 100 | > 100 | – | > 100 | – | |
| Balm | IC50 | > 100a | > 100a | > 100a | – | > 100 | – |
| GI50 | > 100 | > 100 | > 100 | – | > 100 | – | |
| TGI | > 100 | > 100 | > 100 | – | > 100 | – | |
| LC50 | > 100 | > 100 | > 100 | – | > 100 | – | |
| Yarrow | IC50 | 31,4 ± 8,5a | 48,5 ± 2,5a | > 100 | 28,8 ± 15,8 | > 100 | > 500 |
| GI50 | 37,4 ± 7,3 | > 100 | > 100 | 49,4 ± 25,7 | > 100 | > 500 | |
| TGI | 56 ± 1,4 | 65,1 ± 6,1 | > 100 | 86,6 ± 3,4 | > 100 | > 500 | |
| LC50 | 70,6 ± 7,1 | > 100 | > 100 | > 100 | > 100 | > 500 | |
| Marigold | IC50 | 39,8 ± 4,6a | > 100a | > 100a | – | > 100 | > 500 |
| GI50 | – | > 100 | > 100 | – | > 100 | > 500 | |
| TGI | 54, ± 3,1 | > 100 | > 100 | – | > 100 | > 500 | |
| LC50 | 78,5 ± 1,4 | > 100 | > 100 | – | > 100 | > 500 | |
Data (> 100): not significant activity found at 100 μg/mL concentration; (−): not determined; SFE Supercritical Fluid Extraction, UAE Ultrasound Assisted Extraction, RVM Residual Vegetable Matrix, OVM Original Vegetable Matrix. aData acquired from a previous work [4]
Data are presented as IC50 (μg/mL) (concentration needed to induce 50% cell viability inhibition [4, 5] <sup> 5</sup>, GI50 (μg/mL) (concentration needed for 50% growth inhibition), TGI (μg/mL) (concentration needed for total growth inhibition) and LC50 (μg/mL) (concentration needed for 50% cell death) after 48 h treatment as mean ± SEM of at least three independent experiments each performed in quadruplicate