Table 2.
Concentration parameters of the selected plant extracts on PANC-1 cell line sensitivity depending on the extraction method
| Extraction Method | ||||
|---|---|---|---|---|
| SFE | UAE | Commercial | ||
| Concentration Parameter | OVM | |||
| Plant extract | Ethanol | |||
| Marjoram | IC50 | > 100 | – | – |
| GI50 | > 100 | – | – | |
| TGI | > 100 | – | – | |
| LC50 | > 100 | – | – | |
| Yarrow | IC50 | > 100 | > 100 | > 500 |
| GI50 | 24,5 ± 0,26 | > 100 | > 500 | |
| TGI | 96,4 ± 6,2 | 69,2 ± 1,1 | > 500 | |
| LC50 | > 100 | > 100 | > 500 | |
| Marigold | IC50 | 43,2 ± 7,9 | – | > 500 |
| GI50 | > 100 | – | > 500 | |
| TGI | 45,9 ± 1,8 | – | > 500 | |
| LC50 | 74,2 ± 6,6 | – | > 500 | |
Data (> 100): not significant activity found at 100 μg/mL concentration; (−): not determined; SFE Supercritical Fluid Extraction, UAE Ultrasound Assisted Extraction, OVM Original Vegetable Matrix
Data are presented as IC50 (μg/mL) (concentration needed for 50% inhibition of cell proliferation), GI50 (μg/mL) (concentration needed for 50% growth inhibition), TGI (μg/mL) (concentration needed for total growth inhibition) and LC50 (μg/mL) (concentration needed for 50% cell death) after 48 h’ treatment as mean ± SEM of at least three independent experiments each performed in quadruplicate