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. 2018 Sep 17;18:249. doi: 10.1186/s12886-018-0886-z

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Characterization of rat retinal microglia and RMECS. A: Cultures of primary isolated microglia. B: The purity of isolated microglia detected by low cytometry through its specific surface marker CD11b is 93.6%. C: Culture of RMECs. D1-D3: Immunofluorescent detection of RMECs marked by vWF, DAPI and both. E1–E4: Normal microglia are static. F1 − F4: Exposure to 100 ng/mL LPS for 24 h altered the morphology of the microglia from a ramified state with long processes to the activated state with an amoeboid appearance. E1-E4, F1-F4 has been published in our previous research. Abbreviations: vWF, von-Willebrand factor; LPS, lipopolysaccharide. (Page9–10)