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. 2018 Sep 17;18:249. doi: 10.1186/s12886-018-0886-z

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Effects of static or activated microglia on tube formation of RMECs. a-c One representative experiment of tube formation of three different groups at 4 h. d-g Total tube length, number of nodes, number of branches, total branching length quantified by ImageJ. Both static and LPS-activated microglia stimulated tube formation in the RMECs. In addition, the effects on RMEC tube formation were enhanced by the activated microglia compared with the static microglia. Each bar graph indicates means ± SD of three independent experiments. Significant difference in results between the two compared groups. *P < 0.05 using one-way ANOVA. **P < 0.01 using one-way ANOVA. Abbreviations: Con, control RMECs (i.e., cultured without microglia); MG, REMCs co-cultured with static microglia; LPS-MG, REMCs co-cultured with activated microglia; LPS, lipopolysaccharide. (Page 11, paragraph 3)