Table 2.

Primer sequences and condition used for PCR and Sanger sequencing of UBIAD1 gene

Target	Forward primer	Reverse Primer	Size (bp)	Enzyme	Annealing Temp
exon 1	CCGTCCTTCCTCCTTCCC	AAGCCACCTTTGACATCCCT	700	GoTaqGreen	65 °C
exon 2	CCACCTGCACAGTCTAAGGA	CTGCCAAATCACATTCCTTCCT	689	GoTaqGreen	60 °C