Table 2.

Metrics of the A. vulgare metagenome assemblies

Origin	N	Gender	Wolbachia	Tissues	No. of reads	No. of contigs	N50	% reads used	No. of predicted ORFs1	No. of LDC*
Laboratory	7	M	–	Tissue samples*	93,016,946	545,551	575	79.3	173,550	1
Laboratory	7	M	–	Hindgut	151,479,496	862,485	745	85.2	300,044	1
Laboratory	7	M	–	Gut content	44,025,120	181,858	490	60.5	77,326	135
Laboratory	8	F	–	Tissue samples*	115,690,244	617,032	638	82.6	198,980	0
Laboratory	8	F	–	Hindgut	213,961,342	919,075	727	86.1	309,351	4
Laboratory	8	F	–	Gut content	85,069,086	460,039	516	70	165,226	92
Laboratory	7	F	+	Tissue samples*	136,360,100	714,448	647	84.5	238,023	32
Laboratory	7	F	+	Hindgut	196,487,170	960,311	760	90	321,849	6
Laboratory	7	F	+	Gut content	61,471,292	284,429	502	65.1	101,524	72
Availles	10	M	–	Tissue samples*	133,995,390	708,519	707	85.7	229,060	2
Availles	10	M	–	Hindgut	211,001,880	857,462	747	87.7	286,800	7
Availles	10	M	–	Gut content	36,755,870	19,887	513	10.1	20,111	46
Availles	10	F	+	Tissue samples*	144,043,638	692,481	658	85.7	230,975	13
Availles	10	F	+	Hindgut	212,185,430	920,773	814	90.4	314,895	17
Availles	10	F	+	Gut content	55,605,558	200,488	452	52.7	67,468	36

LDC, lignocellulose-degrading CAZymes

*Tissue samples: caeca, nerve cords, gonads, and hemolymph

¹ORFs, predicted ORFs after filtering